Albatross, you seem to be asking 2 different questions:
1. How to calculate load resistance when you double the number of tubes? Shooter gave the answer. Divide by the number of tubes, in this case, two.
2. How to calculate load impedance from plate impedance?
a) you don't. The tube manufacturer does that for you.
b) If you must, the simple thing is that for a voltage source (as opposed to a current source), the input impedance should be 10X the output impedance. Even a power tube may be considered a voltage source. It takes an OT to transform the power tube's output into a decent amount of current. So, to the OT primary, the power tube looks like a voltage source. Hence, in theory the OT primary impedance should be 10X the output impedance of the power tube(s). (Of course, the OT's primary impedance is a reflection of the speaker load impedance back through the OT's windings.)
But it's not that simple in practice, because sound is involved. Distortion is another factor in play. In the tube charts, the manufacturer is also giving you a distortion figure along with plate voltage and load impedance figures. Measuring distortion requires specialized equipment and expertise.
Meanwhile, measuring plate impedance is essentially impossible for the ordinary mortal. We need the manufacturer to specify this for us.