As for the output transformer (someone help me with the math):
You have two optional OTs: a 10K:150 and a 10K:600
So a 10K:600ohm is a 4:1 transformer in terms of winding count. If its to plug into a house PA or recording console, you'll want 'pro audio' line level, which means between +4dBu and maybe +6dBu is a good target to aim for (I've seen some devices with a max. input of +25dBu, which is pretty huge!). So, Maybe aim for 4v p-p? working backwards through the OT, you want a 16v p-p signal on the primary side.
BTW: is the 10K:150 really the same 4:1 / 10K:600CT with the two secondaries strapped in parallel to make it a 150ohm? If so, it still 4:1 OT, and you'd still want 16v p-p, if ts really a 10K:150, its an 8:1 and you'd want 32v p-p on the primary. big difference.
Lets stick with a 4:1, 16v p-p is a low expectation that part of the ciruit, If its 40v p-p on the primary, then you'd get
10v on the sec, or about +12dBu, which might still be okay.. whats the p-p signal voltage in that part of the circuit?
If you always play the amp at max volume, you can set the Lo-Z's p-p voltage based on some set two-resistor voltage divider coming off signal en route to the PI. If you want to use the amp at low volume sometimes, and high volumes other times, you might want to make that voltage divider a pot so you can turn down or turn up the lo-Z's output.
Is this too simple? replace a 1M grid-leak resistor with this 1M pot? or replace that switch you have between the .22 and .022 on the PI with this?

If too simple, there here are two others I have in my notebook (first is mostly from a Jensen whitepaper):

and

also,
I'd also switch that entire FX loop out from the first 12au7 section's grid to the second section's plate coupling cap out.., If you aren't using the FX loop, you can take two triodes and 4 caps out of the signal's path, and really Keep It Simple.
Also, the FX send-level pot is superfluous right? In both cases of "12AY7" or "12AY7+5751" there is a level pot immediately before the FX buffer...