Okay,
This is what I understand. You Power up the amp. As soon as you take it off standby, the fuse blows, even with
all tubes removed. No immediate evidence of any modifications.
Probably safe to say it is not the power transformer.
For your amp , the problem is in your power supply this include output transformer . Any modifications before , in the amp ?
Now, The FIRST place I would check is the 4 diodes that make up the bridge rectifier. One blown diode (to a short condition, not open) would blow your primary fuse. Check all four and let us know. I presume you have a multimeter.
I think we can safely eliminate the output transformer, at least with a simple test anyway. Besides, if that 315mA HT fuse hasn't blown, I wouldn't look past the first place I mentioned as I find it doubtful that the output transformer is the problem. There is a fast way to make sure though and it won't cost you anything but time. Test all the primary leads of the output transformer for a short to ground. If those leads (typically red, blue, and brown on newer amps at least) show a path to ground, then it is possible for it to be a bad output transformer.
[ EDIT ]
Be careful of any high voltage left inside of the amp. Disconnect the wire from C400 where it connects to the diodes and short it to ground through a resistor. Just remember to hook it back up when you're done.