the bias splitter resistors
That's not a term I would use. Those resistors are the output tube grid return resistors. Those resistors provide a path to the output tube cathode thru the bias supply, ground, and finally to the cathodes. There must be a dc path between the grid and cathode for biasing to be valid.
The grid return resistors do impact the PI tube. Each resistor is in parallel with the plate load resistor and are part of the total load on the PI tube. Smaller resistors will load the PI more. Larger resistors will put a lighter load on the PI.
And those resistors also interact with the coupling caps just like the grid resistor interacts with the coupling cap in any RC coupled amp. There is an RC time constant that is determined by multiplying the resistance times the capacitance. Larger time constants can pass lower frequencies. IE, you can make the bass response greater by increasing
EITHER the coupling cap value
OR the resistor value. The opposite is also true.
However, there is no longer this 300K resistance across the PI outputs or the power tube grids since it has now been split.
Those resistors are not in series as far as the PI or output tubes is concerned. The junction of those resistors is at AC ground via the bias cap.
Splitting those resistors will have verrry little effect on either the PI or output tubes. This assumes the bias circuit below those resistors does not change drastically and remains the same for each separate bias adjust circuit. A drastic change would be something like changing the 22K bias pot to a 100K or 250K pot.