> harmonica... I put out a bit more volume than the keyboard did...
Put your ear INside the organ chamber. It is a lot louder in there than in the room.
Sluckey's advice to use a 1.5k cathode resistor is spot-on conventional, and WILL work, though other circuit changes may be suggested (like a volume control after this stage to adapt to the much larger dynamic range of pop/rock guitar versus $29 parlor organ).
> questioned the biasing for V1A. R1 seems too low to be very effective for grid leak bias.
I got wondering. By inspection, it runs real close to the zero-grid line. Approximately -0.2V Vg1. (Peak plate swing OTOO 10V.)
Do we get 0.2V from a 220k grid resistor? IS it a 220k? There is a microphone. What kind? In that day/price, either a crystal or a dynamic. The crystal IS a capacitor, avoiding your objection that gridleak needs a cap to not suck-off bias. A dynamic would surely be <1k, so grid really should be AT 0.0V. IMHO a crystal is more likely...
AH! "CM 390k microphone resistor". Makes no sense with dynamic. On a crystal, this (with 220k) sets a high-pass. You really want a high-pass on a mike inside a reed-organ chamber, there's lots of low-freq turbulence from the blower, enough to overload the amplifier with blower-rumble.
So the gridleak is 220k|390k= 141k. Apparent grid bias is 0.2V. 0.2V/141k is 1.4 microAmps. This is not an unlikely value for small triode at -0.2V. 12AX7 grid current varies a LOT between brands and vintages. In this zone, easily 10:1. Grid wire smoothness, alignment, coating, gas/ion content. I believe vintage 12AX7 controlled this approximately; tubes made after gridleak fell out of fashion may have a much wider range of grid current.