A standard 6-string 21-fret guitar fretboard in standard tuning goes up to about 1kHz. So it's possibly a harmonic resonation of that.
Have you checked for
1) a microphonic tube?
2) a slightly loose input jack or FX-loop jack tip-switch contact?
Well I swapped each tube one at a time, tried different type P.I. tubes, 12AY7, 5751, etc, no change.
Before I retired I maintained Navcomm, communications and some radar systems for the FAA. One thing I learned was when you have a problem, sometimes you have to regroup and start over. It’s easy to go down the wrong path and miss something.
I decided to try this, my other amp, a modded Marshall 2203 has an almost identical preamp, only different is the grounding scheme and wiring dressing. Other differences are I’m using a discrete power chip to drive and 2N2222A to recover reverb instead of a 12AT7 & transformer. Same reverb tank being used in both. The last difference are a pair of EHKT90’s instead of the 6CA7/EL34’s and slight difference in power tube grid resistor size, 120k instead of 150k.
Ok, I wanted to eliminate the output power section as the cause so I connected a short cable from 4210 FX Loop send to 2203 return using just the preamp up to the FX Loop of the 4210 and power amp section of the 2203 (this includes V4A/B too and reverb). Result? No change.
Ok, now I reversed the connection scheme and used the 4210 power section and 2203 preamp section via FX Loop send & return. Result? No real change but slightly a little less ringing but that could just be the EQ settings. So either both amps are fooked or it’s the cabinet.
I’m now focusing on the speaker cabinet. I used the combo open cabinet with both amps on the bench. It’s a 12 inch Celestion T3135 / 8 ohm G125 - 125W speaker. I have the extension cabinet for that combo too with the identical speaker.
Tomorrow just for chits and giggles I’ll repeat the test only with the extension closed back cabinet. My hope is it’s the open back combo only and then I can work it out from there.
I’m also reinstalling the 33k DC elevate R2 resistor. 50vdc elevate is good enough and works well. Just because Kevin O’Conner thinks you need 70 ~ 80v doesn’t always makes it so….