Looks really good overall, especially for a first scratch build.
Having the bias supply in the middle of the board is a bit unusual, but it seems to conserve space here nicely. My only concern would be having raw AC from the PT secondary in that general vicinity. Is there any way you can squeeze the "range resistor" and bias diode down at the PT end of the board, then float the connection to the bias filters "above" the board?
This is mostly personal style, but I would tuck the heater wires into the bottom edge of the chassis away from the board to keep them on the chassis and away from everything else. Also, are the PT heater leads tied down between the positive ends of the first 2 filter caps? I'm assuming the two green PT leads are for the heaters but obviously could be wrong. If not, why can you run the green PT leads straight to the first power tube?
Plate leads should lie flat on the chassis, perpendicular to and away from everything else. Leads going to control grids are especially sensitive, so I'd have them come straight off the board (up from the board's perspective) and then down to the tube sockets. Cathodes aren't as sensitive but I tend to keep them floating as well. Note that only works if you've moved the heaters to the inside lip of the chassis.
It took me a few builds to really start thinking about lead dress in three dimensions but that can help a lot IMHO.
One safety note: power cord wiring should always be done so that the green safety ground would be the LAST thing to break free. IOW you need more slack in the green lead of the safety ground than in the hot or neutral wires. Can't tell from the pics if you've got that there.
Sorry if this seems like nit-picking... you did ask for it!

Congratulations on the fully functional build!
Cheers,
Chip