Not to toot my own horn, but...
If a signal source was available, applied to the input and you measured signal at each stage, you would have found quickly that everything from the input jack to the output tubes seemed good. You could even use the estimated amp wattage and the speaker impedance to guesstimate a proper speaker-level voltage, and measuring the OT secondary for that voltage would have confirmed that the OT was working correctly.
With input jack to OT secondary ruled out as the problem, the only thing left would be the jack and the speaker itself.
It been a long time since I repaired amps. I should sit down and think up a logical way to run through checking out a malfunctioning amp in the least amount of time. There are people out there who already know how to do this, but it's never written down anywhere, and their personal experience with certain models and certain failure modes can lead them to the problem with very little time spent troubleshooting.
If you don't already do it, I'd recommend keeping a logbook of amps that you've worked on. Document the observed malfunction/symptom(s). Also document the model, year, modifications, circuit type, etc. Keep notes stating what you tried with the amp to fix it (espeically the stuff that didin't work). And of course, record what the final found problem was. If you do this, you might notice either a pattern of problems with a given model, circuit, or construction type that could lead you to a more efficient way of testing that type of amp. It might also point out where your troubleshooting methods need to be revised to save time.