I've been where you're at a few times. You have the best advice here that you can get.
Do all the things mentioned, especially the insulated clips on your meter like Willabe mentioned.
I did all my first few builds using a lay out, they were easier builds, like a 5E3. I got lucky the first time and the amp played great. I though, boy this is easy till the next build I had problems like you're having now. So this is normal.
If you don't understand the schematic and can't relate to the wiring by looking at the schematic, use the layout. And by all means double check every connection (and resistor value with your meter) just before before soldering, it will be slow but worth it. Once soldered make the mark on your layout and on the bd. a dot with a sharpie (non-permanent). This will help make sure your not making mistakes.
Another thing is the tendency to wire things backwards, like pots. So position your layout next to the build exactly the same and follow.
Now when your done and if the amp doesn't have the correct voltages don't start over. Go slow and recheck everything. It's hard to check the values of resistors wired in a circuit, so do what Sluckey recommended, measure the resistor before you solder it in place.
You could be having "cold solder joints or burning up components, don't leave the tip on a connection more than five seconds (hope you have the right tip). Heat both the lead and turret at the same time for about three/four seconds and then apply the solder to the lead not the tip, it should flow and be shinny when first done.
I still have some problems reading some schematics but I find I can now better repair or build an amp by the schematic than a layout.
Believe me you'll get it going, and remember you're dealing with high voltage that can hurt you so be careful.
Just the other day, I put a 7591 power tube NOS, in the 5Y3 rectifier socket and turned on the amp. I mixed the two tubes. After a few minutes trying to find out why I had no sound I saw the problem. But I fired a perfectly match NOS Westinghouse 7591.

We all still make mistakes, I'm still leaning.
Go slow, and keep posting.
al