One thing seems clear: You haven't jumpered the two cathodes on V6, the last tube. They should be connected common, on the socket, with a piece of bare wire. (Or however you wish to do it, but that's how it's generally done) You have one plate of that tube at full B+ (500v) which means that it is drawing zero current, and that's sucking up the cathode. Those two cathodes have to be the same voltage: they are hard wired together (or, should be)
Then you have something else going on with your tremolo tube, V5. You understand that a 12a_7 is two identical triodes, so, it is a matter of choice whether you happen to use the triode represented by pins 1-2-3 for one thing and the triode represented by pins 6-7-8 for another or vice versa. As long as you were coherent, it would not matter. What I am getting at is, regardless of that/those choices, the cathodes (pins 3 and 8) of V5 sit atop those paralleled res-cap structures and both of them should be sitting at single-digit volts. 2-3-4 volts, closer to 2, usually. You show 248 volts on pin 8 of that tube. No way. Pin 8 is a cathode. You should have 2-3 volts on it. Either side of that tube should have 2-3 volts on the cathodes It must not be connected to ground in its own private way.