1. Nomenclature clarification. Gain is always a multiplier. So, Zero gain = No output, because any number X 0 = 0. If signal strength input is equal to signal strength output, that's Unity Gain. The multiplier for Unity Gain is "1"; hence the name "unity" (a unit = 1). Any number X 1 = that same number.
There is another way to speak in terms of signal strength: Decibels (dB). So, another way of saying "Unity Gain" is "Zero dB of Gain". That's the way to talk about "0" , when signal-in = signal-out.
Unfortunately the only way to really get into the theory of gain is to learn loadlines. I found it to be a painful learning curve, just to get the jist of it. The patient help of Dummyload, Sluckey PRR & Hotblue was invaluable!
2. Simplicity. A 12ax7 has a nominal gain factor of 100; A 12at7, 60. That's a difference of 40%. A cathode bypass cap approx. doubles the gain of a tube, a difference of 50% which is in the ballpark. So switching out the bypass cap on a 12ax7 stage will sorta make it perform like a lower mu tube.
You can do more radical things to mess with the gain of a 12ax7 tube. But again, if you force it to perform at only 60% of its gain potential, then it will will simulate a 12at7, but only in terms of the gain factor. The loadlines & operating points will probably differ significantly for a 12ax7 vs. a 12at7 at such operating points. So tone, in terms of saturation, clipping and harmonic distortion generally, may be significantly different.
Though gain is important, it is not the only, nor the most important factor. Any tube type produces far more gain than we need. The most important factor is Tone, which derives from the operating point.