Original Question:
Where do you set the volume and tone controls when biasing?
Tone controls don't matter, but set the volume at 0.
... when I started turning the volume and tone controls as well as the presence control the bias starts going down like from 35milli amps to 25 and 20.
When you're measuring, you're checking direct current (or voltage across a resistor due to direct current).
When you add a signal (even background hiss due to turning up volume with not signal input) you no longer have direct current at the output tubes but an alternating current bobbling around some d.c. midpoint.
When you measure d.c. when there's really a.c. present (and possibly a very random a.c. due to noise), there's no telling what your meter will display. It depends on if you're using a digital or analog meter & the details of exactly how the meter does the actual measurement in d.c. mode. The latter indicates whether the meter can measure d.c. while rejecting a.c. also present at the measurement point.
Digital meters sample the voltage so many times per second. For d.c., maybe the meter displays each sampled voltage (not likely, because the display would be switching very fast between each measured value). Maybe the meter averages the measures for a certain time period. Even if it averages, there are also discrete samples used for the average, and those could land more on the negative end of the waveform and drive the average downward. Some meters can reject a.c. present very well. Some can only reject a.c. if it is a repetitive waveform (like that from a signal generator, not random noise). Some claim to reject a.c., but only reject well at 50/60Hz.
The best answer is don't set up the conditions for the test equipment to lie to you. Don't have a.c. present for a d.c. measurement. Turn the volume down. If background noise is really an issue, pull the phase inverter tube from its socket.