The greatest possibly IMO is the failure of an electrolytic cap or, one of your tubes developed a heater-to-cathode short. Both of those are pretty rare, but not impossibly rare, and definitely not impossible.
You say the amp was working OK, then all you did was to change speakers, in other words, you touched nothing inside the chassis, moved no wires (although the insertion of a 1/4" plug into the speaker makes a tiny movement of "things") and made no ckt changes.
Yes, it frustrating to try to diagnose things with no test gear nor spare parts. It's voodoo.
Here are some ideas to try.
1: You have tried pulling tubes starting from the preamp end of the amp. That is somewhat logical, but it's not the case that the input guitar signal goes thru V1, then V2, then V3, then V4. You say when V1 and V2 gone = no change. But pulling V1,2 AND V3 made the hum go away. Now, I find that telling, because V3 is the reverb driver. Only a *part* of the full signal goes thru it. The amp should still work (without reverb of course) lacking a V3. Does it? IOW, you should be able to pull V3 and be able to play a non-reverb amp using either Ch1 or ch 2 with no hum. A good thing to test. >>Pull only V3<< and try the amp.
2: The immediate suspect has to be the 12AT7 in V3. This tube is fed very high volts and is driven hard in any reverb-Fender and if you know your Fender blackface amps, you know this tube gets really hot, much hotter than the others. I am thus pointed to a H-K short in V3. Maybe this tube suffered an early death. The amp does not NEED V3 to work. But V3 can certainly induce hum if it has an internal H-K short. If the hum goes away with that tube and only that tube pulled, suspicion grows > H-K short V3.
3: *IF* you can pull V3 and have a working amp (with no reverb) get the reverb tank cabled in. Power up the amp and shake the reverb can a little. The reverb control has to be off zero, set it to 3-4-5. Do you hear crashing when you move the reverb can?
4: You say you have swapped V1 and V2. These are ch1 and ch 2 preamp tubes. The next thing to do is to pull V2 and place it into V3 socket. (The amp now has all tubes installed except for V2, and V3 is replaced by a 12AX7 instead of the normal 12AT7) Now, (a) see if hum goes away, and (b) see if you have reverb. The 12AX7 should work fine as the reverb driver (you may get a lot more 'verb than normal, we would expect that from the higher gain 12AX7 in place of the mid-gain 12AT7)
I again lean toward a H-K short in V3. Bad tube.