With a 12AX7, I don't think that can happen. with that kind of B+, what you might get from a 12AX7 gain stage: with a bigger 270k plate resistor (Rp) --and assuming a small cathode resistor (Rk) 1.5K-2.7k-- you'd probably see 60V @plate; with Rp=100k, you'd probably see 120V @plate. If you are driving a tone-stack, I don't think you'd want to go lower than a Rp=100k (output impedance of the stage goes down as the Rp goes down). Even if you do try to go lower, like Rp=47k, you might see 150V? any lower and you are entering current-driver territory, and not gain producing voltage-drivers. and these voltage guesses are optimistic..
things to consider:
is this for bass or guitar? you want clean or overdrive and distortion?
if you are planning to plug into the 'power amp in' on a Sunn amp, you'll need a lot of clean gain, so you'll need a lot more B+. you also need to see what the input impedance (Z) of that input is, and make sure your output Z is appropriate.
If you are plugging into the instrument input and all you really want is some boost, then you can probably do something with that PT. a simple 12AX7 gain stage with a volume knob work work. You could add a single one knob treble roll off control if you want. The Vox Top Boost stage is fun and uses the other 1/2 triode as a coupled CF.