When you power up without tubes your voltages will be high, hopefully not above your ecap's rating though.
Also without any load on the PS in many circuits you may find your B+ reading at every PS node, that is OK.
Then you want to check the pins on your sockets.
You should have your heater voltage on the corresponding pins of every socket.
Like for 6V6's pin 2 to 7 should read 6.3VAC or slightly higher unloaded.
12AX7s pins 4 to 9 and 5 to 9 should have that too.
Then the plate and screen pins on the output tubes should show your B+ referenced to ground.
The plates of your preamp tubes may also show B+ referenced to GND.
As long as you have some voltage readings on those plates they should work.
If you have a fixed bias circuit make sure you have adjustable negative voltage measured to Gnd on those pins.
Set your fixed bias reading at the high end of your - voltage range.
That should keep your output tubes from red plating at startup.
Then when you power up with the tubes in you want to see the correct voltages at each PS node.
You can then double check your preamp plates, which should now read their correct voltage referenced to ground.
Then I adjust the fixed bias voltage to the desired level.
I do those things before I try playing the amp.
Any voltage discrepancies should be addressed before you try playing.
