Good point on the filter caps. They're 525 volts. Thankfully.
E- caps can take some over voltage for a little while, but your still stressing them.
Your being over cautious if you 1st tested the rebuild of that amp with just the rectifier tube. Not only do you have a bulb limiter but your also using a variac with it.You can do that but many guys would just use the bulb limiter by itself.
With both the limiter and the variac, even with just the bulb limiter, you could have put all the tubes in and fired it up. Bulb stays lit, just turn it off. Then go back and start pulling 1 tube at a time until that bulb stays dim. Pull a power tube, turn the amp on, look at the bulb, stays bright, turn the amp off, with that 1st tube still out pull the next power tube, etc. When the bulb dims back down again, you found the tube/circuit that's the problem. Or, it might be a direct short in your wiring somewhere else.
If current is not being drawn properly on those tubes, would that affect the "glow"?
No, if the tube is drawing normal current or not, that won't change the filaments glow.
Some tubes are just hard to see the tip/end of the filament because of the way the tube was made.
If so, what would be affecting the current draw?
Probably mis-wired, or bad tube. Check the dcv's at all pins, you know this by now. What do you get?